When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

Digital PCR, or dPCR, is a molecular biology technique that detects and quantifies nucleic acid targets. By partitioning a sample into many individual reactions, scientists can measure whether a ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...

Gene-Analysis Studies Advanced by Use of Low-Volume High-Throughput Real-Time PCR This article describes three gene-analysis studies using the Roche LightCycler® 1536 Real-Time PCR System (Figure 1).

Adaptive PCR cycling and AutoNormalization improve full-length 16S sequencing across diverse microbiome samples and enhance ...